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Handling & Preparation Reconstitute using bacteriostatic water per laboratory protocol Use only trained professionals Follow institutional research and safety standards Storage Instructions To preserve potency and stability: Store lyophilized vials in a cool, dry environment Refrigerate reconstituted solutions at 28C Protect from heat, light, and moisture Avoid repeated freezethaw cycles Possible Observations in Research Settings Reported observations may include: Mild redness at administration site Temporary dizziness or headache Transient nausea All findings should be documented and evaluated within controlled research environments

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For Salmonella infections, immortalized BMDMs were seeded in 12-well plates (4 10 5 cells per well) in RPMI 1640 supplemented with 2 mM L-glutamine and 10% FBS at 37 C and 5% CO 2 for 24 h